Biomol/CRISPRa (SAM) Jurkat Cell Pool/2 MCells/BPS-78080
市场价：
¥19960.00
美元价：
9980.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product information "CRISPRa (SAM) Jurkat Cell Pool"
This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-kappa-B p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2-tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene.
| Keywords: | SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1 |
| Supplier: | BPS Bioscience |
| Supplier-Nr: | 78080 |
Properties
| Application: | Quick knock-out cell pool / cell line generation, SgRNA screening |
| Reactivity: | Human |
Database Information
| KEGG ID : | K09952 | Find alternatives |
| UniProt ID : | Q99ZW2 | Find alternatives |
Handling & Safety
| Storage: | Liquid nitrogen (store in liquid nitrogen) |
| Shipping: | -80°C (International: °C) |
Caution Our products are for laboratory research use only: Not for administration to humans!
品牌介绍
抗体在Biomol，您将找到适合您的研究的正确的多克隆或单克隆抗体。选择偶联（标记）的一抗（例如，FITC和生物素）用于抗原的直接免疫检测，或一抗加上二抗用于间接抗原检测。免疫球蛋白G类（IgG）的抗体主要用于免疫学检测。它们由四个蛋白质链组成：两条分子量为25 kDa的轻链（L）和两条分子量分别为50 kDa的重链（H）。由二硫键连接的重链和轻链（H + L）一起形成了众所周知的抗体结构。对于某些实验，这些免疫球蛋白的酶消化产生具有优势性质的抗体片段。
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