Biomol/NF-kappaB（p65）转录因子分析试剂盒/96孔/Cay10007889-96
商品编号：
Cay10007889-96
品牌：
Biomol
市场价：
¥10640.00
美元价：
5320.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product information "NF-kappaB (p65) Transcription Factor Assay Kit"
Cayman"s NF-kappaB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kappaB response element is immobilized to the wells of a 96-well plate. NF-kappaB contained in a nuclear extract, binds specifically to the NF-kappaB response element. NF-kappaB (p65) is detected by addition of specific primary antibody directed against NF-kappaB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman"s NF-kappaB (p65) Transcription Factor Assay detects human NF-kappaB (p65). It will not cross-react with NF-kappaB (p50).
| Keywords: | RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Supplier: | Cayman Chemical |
| Supplier-Nr: | 10007889-96 |
Properties
| Application: | NF-kappaB binding detection |
| Reactivity: | Human, Mouse, Rat |
| Format: | Solid Phase |
Database Information
| KEGG ID : | K04735 | Find alternatives |
| UniProt ID : | Q04206 | Find alternatives |
Handling & Safety
| Storage: | -80°C |
| Shipping: | -20°C (International: -80°C) |
| Signal Word: | Danger |
| GHS Hazard Pictograms: |   |
| H Phrases: | H314, H335 |
| P Phrases: | P260, P261, P264, P271, P280, P310, P312, P321, P363, P301+P330+P331, P303+P361+P353, P304+P340, P305+P351+P338, P332+P313, P337+P313, P405, P403+P233, P501 |
Caution Our products are for laboratory research use only: Not for administration to humans!
品牌介绍
抗体在Biomol，您将找到适合您的研究的正确的多克隆或单克隆抗体。选择偶联（标记）的一抗（例如，FITC和生物素）用于抗原的直接免疫检测，或一抗加上二抗用于间接抗原检测。免疫球蛋白G类（IgG）的抗体主要用于免疫学检测。它们由四个蛋白质链组成：两条分子量为25 kDa的轻链（L）和两条分子量分别为50 kDa的重链（H）。由二硫键连接的重链和轻链（H + L）一起形成了众所周知的抗体结构。对于某些实验，这些免疫球蛋白的酶消化产生具有优势性质的抗体片段。
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